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Improving the precision of fluorescence lifetime measurement using a streak camera
作者: 李恒, 邵永红, 王岩, 屈军乐, 牛憨笨 主题: 年份: 2010
摘要: streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. however, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. we have constructed a two-photon excitation fluorescence lifetime measurement system that is based on a synchroscan streak camera and have developed accordingly a method to correct the effect of gain nonuniformity and nonlinearity of sweeping speed on the measurement precision. a continuous-wave laser of high stability is used to calibrate the gain of the streak camera, and a fabry-perot etalon is used to calibrate the nonlinearity of the sweeping speed. fitting algorithms are used to correct the gain of the streak camera and nonlinearity of the sweeping speed respectively, which significantly improves the measurement precision o...
A miniature laser speckle fluorescence sectioning microscope for cell imaging
作者: 邵永红, 李恒, 文侨, 王岩, 屈军乐, 牛憨笨 主题: 年份: 2010
摘要: we present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state (dpss) laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. high contrast fluorescence sectioning images of bovine pulmonary artery endothelial (bpae) cells are obtained. the image quality is similar to that of the images acquired by standard laser scanning confocal microscope (lscm). compared with lscm, the laser speckle fluorescence microscope (lsfm) presented in this letter has many advantages, such as simple configurations, low cost, compact, and easy to operate, which makes it possible to have wide spread applications in biomedicine.
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